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primary rabbit anti-eea1 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primary rabbit anti-eea1 antibody
    Primary Rabbit Anti Eea1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+anti-eea1+antibody/rabbit+anti+eea1/pm37368691-322-16-19
    Average 90 stars, based on 1 article reviews
    primary rabbit anti-eea1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Incubation:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Translocation Assay:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    MTS Assay:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Comparison:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Super-Resolution Microscopy:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Microscopy:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Blocking Assay:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Derivative Assay:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Ex Vivo:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Modification:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Functional Assay:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Membrane:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Western Blot:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Marker:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Cell Fractionation:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Fluorescence:

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 °C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.
    Article Snippet: The samples were incubated overnight at 4 ◦C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells
    Article Snippet: For immunohistological staining, the cells were incubated at 4 °C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells.
    Article Snippet: For immunohistological staining, the cells were incubated at 4 ◦C overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg Germany) in 1:10 diluted permeabilization/blocking solution.

    Article Title: Clostridium botulinum C3 Toxin for Selective Delivery of Cargo into Dendritic Cells and Macrophages.
    Article Snippet: After washing the cells three times with PBS, the cells were permeabilized and blocked in 3% BSA and 0.3% TritonX-100 in PBS for 2 h. The samples were incubated overnight with 1 μg/mL of primary rabbit anti-EEA1 antibody (Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL Atto594-conjugated GFP-booster nanobody (Chromotek, Planegg-Martinsried, Germany) in 1:10 diluted blocking solution at 4 ◦C.

    Article Title: Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
    Article Snippet: The samples were incubated overnight at 4 °C with 1 μg/mL of the primary rabbit anti-EEA1 antibody (1 mg/mL from Thermo Scientific, Waltham, MA, USA) and 0.5 μg/mL of Atto594-conjugated GFP-booster nanobody (0.5 mg/mL from Chromotek, Planegg-Martinsried, Germany) dissolved in 1:10 diluted blocking solution.



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